Single Nucleotide Patch Base Excision Repair Is the Major Pathway for Removal of Thymine Glycol from DNA in Human Cell Extracts
作者： Grigory L. Dianov , Tanja Thybo , Irina I. Dianova , Leonora J. Lipinski , Vilhelm A. Bohr
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摘要: The repair pathways involved in the removal of thymine glycol (TG) from DNA by human cell extracts have been examined. Closed circular constructs containing a single TG at defined site were used as substrates to determine patch size generated afterin vitro extracts. Restriction analysis incorporation vicinity lesion indicated that majority was repaired through base excision (BER) pathways. Repair 5′ lesion, characteristic for nucleotide pathway, not found. More than 80% accomplished single-nucleotide mechanism, and remaining TGs removed long BER pathway. We also analyzed role xeroderma pigmentosum, complementation group G (XPG) protein step BER. Cell deficient XPG had an average 25% reduction incision. These data show is primary pathway may be accessory factor.
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