The biotin repressor: modulation of allostery by corepressor analogs.

摘要: The Escherichia coli biotin repressor functions in retention and regulation of biosynthesis. Biotin is accomplished via the two-step biotinylation biotin-dependent enzyme, acetyl-CoA carboxylase. In first step this reaction substrates ATP are utilized synthesis activated biotin, biotinyl-5'-AMP, while second transferred to a unique lysine residue carboxyl carrier protein subunit Regulation biosynthesis through binding transcription control region biosynthetic operon. adenylated or as corepressor DNA process. mixed anhydride thus labile. efforts develop tools for structural thermodynamic studies regulatory interactions, two analogs adenylate, sulfamoyl derivative an ester derivative, have been synthesized functionally characterized. Results fluorescence measurements indicate that both bind with high affinity inactive transfer acceptor protein. Functional their properties weak allosteric activator, closely mimics physiological activation assembly repression complex. these also provide further insight into mechanism repressor.