Cloning and in silico analysis of a gene encoding a putative β-carbonic anhydrase from cowpea (Vigna unguiculata L. Walp)
作者： Tapan Kumar Mondal , Showkat Ahmad Ganie , Ravi Ranjan Kumar Niraj , Mukesh Kumar Rana
DOI: 10.1080/17429145.2013.861027
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摘要: A gene encoding putative β-carbonic anhydrase (CA) from cowpea (Vigna unguiculata L. Walp.) was characterized and designated as VuCA1 (GenBank ID: JQ429799). While the genomic sequence of CA found to be 1470 bp long with 4 introns, open reading frame 990 in length. The deduced amino acid contained two characteristic conserved domains, i.e. CSDSRV EYAVLHLKVSNIVVIGHSACG, which showed high degree homology other β-CA genes angiosperms. We have reported three-dimensional model quality predicted analysed PROCHECK. Molecular docking modeled revealed similar binding pockets for different substrates products. expression transcripts detected parts plant, highest level observed leaves, followed by flower buds, weak stems, weakest roots. leaves downregulated zinc deficiency but upregulated salt an...
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