Normal and pathological expression of GFAP promoter elements in transgenic mice.
作者： M. Galou , S. Pournin , D. Ensergueix , J.-L. Ridet , J.-L. Tchélingérian
关键词:
摘要: The expression of the glial fibrillary acidic protein (GFAP), a component astroglial intermediate filaments, is regulated under developmental and pathological conditions. In order to characterize DNA sequences involved in such regulations, we produced transgenic mice bearing 2 kb 5' flanking region murine GFAP gene linked Escherichia coli beta-galactosidase (beta-gal) reporter gene. Seven lines were obtained. We observed that regulatory elements present transgene GFAP-nls-LacZ direct an neural non-neural tissue target vivo unexpected subpopulation astrocyte. developing brain, beta-gal activity appeared simultaneously same region, on embryonic day 18 (E18), suggesting promoter contains responsible for perinatal vimentin/GFAP switch. addition, demonstrated sequence used possess which are activated after surgical injury, thus permitting study some aspects reactive gliosis these mice. These provide useful tool by enabling further studies and, probably, neuronal physiologies.
sci-hub.se 参考文章(51)
ML Voytko, DS Olton, RT Richardson, LK Gorman, JR Tobin, DL Price, Basal forebrain lesions in monkeys disrupt attention but not learning and memory The Journal of Neuroscience. ,vol. 14, pp. 167- 186 ,(1994) , 10.1523/JNEUROSCI.14-01-00167.1994
RH Miller, MC Raff, Fibrous and protoplasmic astrocytes are biochemically and developmentally distinct The Journal of Neuroscience. ,vol. 4, pp. 585- 592 ,(1984) , 10.1523/JNEUROSCI.04-02-00585.1984
Norman Latov, Gajanan Nilaver, Earl A. Zimmerman, William G. Johnson, Ann-Judith Silverman, Richard Defendini, Lucien Cote, Fibrillary astrocytes proliferate in response to brain injury: A study combining immunoperoxidase technique for glial fibrillary acidic protein and radioautography of tritiated thymidine Developmental Biology. ,vol. 72, pp. 381- 384 ,(1979) , 10.1016/0012-1606(79)90127-1
Masayuki Miura, Taka-aki Tamura, Katsuhiko Mikoshiba, Cell-specific expression of the mouse glial fibrillary acidic protein gene: identification of the cis- and trans-acting promoter elements for astrocyte-specific expression. Journal of Neurochemistry. ,vol. 55, pp. 1180- 1188 ,(1990) , 10.1111/J.1471-4159.1990.TB03123.X
Mary E. Hatten, Ronald K. H. Liem, Michael L. Shelanski, Corol A. Mason, Astroglia in CNS injury Glia. ,vol. 4, pp. 233- 243 ,(1991) , 10.1002/GLIA.440040215
G. Le Prince, M.-C. Copin, H. Hardin, M.-F. Belin, J.-P. Bouilloux, M. Tardy, Neuron—glia interactions: effect of serotonin on the astroglial expression of GFAP and of its encoding message Developmental Brain Research. ,vol. 51, pp. 295- 298 ,(1990) , 10.1016/0165-3806(90)90290-F
P. Vijay Sarthy, Marlene Fu, Jing Huang, Developmental expression of the glial fibrillary acidic protein (GFAP) gene in the mouse retina. Cellular and Molecular Neurobiology. ,vol. 11, pp. 623- 637 ,(1991) , 10.1007/BF00741450
Srijata Sarkar, Nicholas J. Cowan, Intragenic sequences affect the expression of the gene encoding glial fibrillary acidic protein. Journal of Neurochemistry. ,vol. 57, pp. 675- 684 ,(1991) , 10.1111/J.1471-4159.1991.TB03799.X
Joanna M. Balcarek, Nicholas J. Cowan, Structure of the mouse glial fibrillary acidic protein gene: implications for the evolution of the intermediate filament multigene family Nucleic Acids Research. ,vol. 13, pp. 5527- 5543 ,(1985) , 10.1093/NAR/13.15.5527
S. A. Reeves, L. J. Helman, A. Allison, M. A. Israel, Molecular cloning and primary structure of human glial fibrillary acidic protein Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 5178- 5182 ,(1989) , 10.1073/PNAS.86.13.5178