Electrotransfer of Plasmid DNA Encoding an Anti-Mouse Endoglin (CD105) shRNA to B16 Melanoma Tumors with Low and High Metastatic Potential Results in Pronounced Anti-Tumor Effects
作者： Tanja Dolinsek , Gregor Sersa , Lara Prosen , Masa Bosnjak , Monika Stimac
关键词:
摘要: Endoglin overexpression is associated with highly proliferative tumor endothelium and also some tumors, including melanoma. Its targeting has anti-tumor effectiveness, which can be obtained by RNA interference. The aim of our study was to explore the effectiveness endoglin silencing electrotransfer plasmid DNA encoding short hairpin against in two murine B16 melanoma variants different metastatic potential on cells, spheroids subcutaneous tumors mice. results demonstrate that gene reduces proliferation, survival migration cells as therapy resulted a high percentage cures (23% 58% B16F1 B16F10 respectively). correlated expression cells; vitro effects were more pronounced express than B16F10. However, opposite observed vivo where there higher better tumor. In conclusion, for treatment seems concept worthy further exploration due increased therapeutic effect based simultaneous vascular its direct cells.
mdpi.com
core.ac.uk
sci-hub.se 参考文章(34)
Richard Heller, Loree C. Heller, Gene electrotransfer clinical trials. Advances in Genetics. ,vol. 89, pp. 235- 262 ,(2015) , 10.1016/BS.ADGEN.2014.10.006
Grant A. McArthur, Combination therapies to inhibit the RAF/MEK/ERK pathway in melanoma: we are not done yet Frontiers in Oncology. ,vol. 5, pp. 161- 161 ,(2015) , 10.3389/FONC.2015.00161
Fatima H. Karzai, Andrea B. Apolo, Liang Cao, Ravi A. Madan, David E. Adelberg, Howard Parnes, David G. McLeod, Nancy Harold, Cody Peer, Yunkai Yu, Yusuke Tomita, Min-Jung Lee, Sunmin Lee, Jane B. Trepel, James L. Gulley, William D. Figg, William L. Dahut, A phase I study of TRC105 anti‐endoglin (CD105) antibody in metastatic castration‐resistant prostate cancer BJUI. ,vol. 116, pp. 546- 555 ,(2015) , 10.1111/BJU.12986
Natasa Tesic, Urska Kamensek, Gregor Sersa, Simona Kranjc, Monika Stimac, Ursa Lampreht, Veronique Preat, Gaelle Vandermeulen, Miha Butinar, Boris Turk, Maja Cemazar, Endoglin (CD105) Silencing Mediated by shRNA Under the Control of Endothelin-1 Promoter for Targeted Gene Therapy of Melanoma Molecular therapy. Nucleic acids. ,vol. 4, ,(2015) , 10.1038/MTNA.2015.12
Anna Niezgoda, Piotr Niezgoda, Rafał Czajkowski, Novel Approaches to Treatment of Advanced Melanoma: A Review on Targeted Therapy and Immunotherapy BioMed Research International. ,vol. 2015, pp. 851387- 851387 ,(2015) , 10.1155/2015/851387
Monika Stimac, Tanja Dolinsek, Ursa Lampreht, Maja Cemazar, Gregor Sersa, None, Gene Electrotransfer of Plasmid with Tissue Specific Promoter Encoding shRNA against Endoglin Exerts Antitumor Efficacy against Murine TS/A Tumors by Vascular Targeted Effects PLOS ONE. ,vol. 10, pp. e0124913- ,(2015) , 10.1371/JOURNAL.PONE.0124913
M Bosnjak, T Dolinsek, M Cemazar, S Kranjc, T Blagus, B Markelc, M Stimac, J Zavrsnik, U Kamensek, L Heller, C Bouquet, B Turk, G Sersa, None, Gene electrotransfer of plasmid AMEP, an integrin-targeted therapy, has antitumor and antiangiogenic action in murine B16 melanoma. Gene Therapy. ,vol. 22, pp. 578- 590 ,(2015) , 10.1038/GT.2015.26
N Nakai, T Kishida, M Shin-Ya, J Imanishi, Y Ueda, S Kishimoto, O Mazda, Therapeutic RNA interference of malignant melanoma by electrotransfer of small interfering RNA targeting Mitf. Gene Therapy. ,vol. 14, pp. 357- 365 ,(2007) , 10.1038/SJ.GT.3302868
G. Dawn, R. M. MacKie, Expression of endoglin in human melanocytic lesions. Clinical and Experimental Dermatology. ,vol. 27, pp. 153- 156 ,(2002) , 10.1046/J.1365-2230.2002.00976.X
Masanori Tsujie, Shima Uneda, Hilda Tsai, Ben Seon, Effective anti-angiogenic therapy of established tumors in mice by naked anti-human endoglin (CD105) antibody: differences in growth rate and therapeutic response between tumors growing at different sites. International Journal of Oncology. ,vol. 29, pp. 1087- 1094 ,(2006) , 10.3892/IJO.29.5.1087