Electrotransfer parameters as a tool for controlled and targeted gene expression in skin.

摘要: Skin is an attractive target for gene electrotransfer. It consists of different cell types that can be transfected, leading to various responses We demonstrate these could controlled by selecting the appropriate electrotransfer parameters. Specifically, application low or high electric pulses, applied multi-electrode array, provided possibility control depth transfection in skin, duration and level expression, as well local systemic distribution transgene. The influence pulse type was first studied using a plasmid encoding reporter (DsRed). Then, plasmids therapeutic genes (IL-12, shRNA against endoglin, melanoma adhesion molecule) were used, their effects on wound healing cutaneous B16F10 tumors investigated. high-voltage pulses resulted expression restricted superficial skin layers induced response. In contrast, low-voltage promoted into deeper layers, resulting prolonged higher transgene production, possibly with distribution. Therefore, translation clinics, it will utmost importance adjust parameters approaches specific mode action gene.