Development and transferability of two multiplexes nSSR in Scots pine (Pinus sylvestris L.)
作者： Stefana Ganea , Sonali S. Ranade , David Hall , Sara Abrahamsson , María Rosario García-Gil
DOI: 10.1007/S11676-015-0042-Z
关键词:
摘要: Single sequence repeat (SSR) multiplexing is a semi high-throughput PCR methodology for the analysis of multiple SSRs. We developed two SSR multiplexes selected from loci previously reported in pine literature and tested transferability both nine other species. 234 nuclear (nSSRs) described ten nSSRs following simple criteria interpretability reproducibility. Selected were divided into multiplex sets their amplification was optimized three different methods based on: (a) custom protocol, (b) protocol with hotstart taq polymerase, (c) commercially available kit multiplexing. To validate performance, level genetic diversity assessed Scots natural populations (Hungary, northern Sweden southern Sweden). In addition, we also these have five each that will contribute to reduce costs nSSR scoring, while increasing capacity analysis. Amplification successful all (94 % success) polymorphism (7.1 alleles/marker) similar populations. Transferability those species closely related pine.
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Shunxue Tang, Venkata K. Kishore, Steven J. Knapp, PCR-multiplexes for a genome-wide framework of simple sequence repeat marker loci in cultivated sunflower Theoretical and Applied Genetics. ,vol. 107, pp. 6- 19 ,(2003) , 10.1007/S00122-003-1233-0
Christian Schlötterer, David B. Goldstein, Microsatellites: Evolution and Applications ,(1999)
M. Morgante, A. Pfeiffer, A. Costacurta, A. M. Olivieri, W. Powell, G. G. Vendramin, J. A. Rafalski, Polymorphic Simple Sequence Repeats in Nuclear and Chloroplast Genomes: Applications to the Population Genetics of Trees Springer, Dordrecht. pp. 233- 238 ,(1996) , 10.1007/978-94-011-3983-0_32
Patrizia Masi, PL Spagnoletti Zeuli, P Donini, Development and analysis of multiplex microsatellite markers sets in common bean ( Phaseolus vulgaris L.) Molecular Breeding. ,vol. 11, pp. 303- 313 ,(2003) , 10.1023/A:1023443109985
M. Shepherd, C. Williams, L. Auckland, Y. Zhou, T. Bui, Conifer microsatellite handbook. Conifer microsatellite handbook.. ,(2002)
L. Tommasini, J. Batley, G. Arnold, R. Cooke, P. Donini, D. Lee, J. Law, C. Lowe, C. Moule, M. Trick, K. Edwards, The development of multiplex simple sequence repeat (SSR) markers to complement distinctness, uniformity and stability testing of rape (Brassica napus L.) varieties. Theoretical and Applied Genetics. ,vol. 106, pp. 1091- 1101 ,(2003) , 10.1007/S00122-002-1125-8
STEFANIE WAGNER, SOPHIE GERBER, RÉMY J. PETIT, Two highly informative dinucleotide SSR multiplexes for the conifer Larix decidua (European larch). Molecular Ecology Resources. ,vol. 12, pp. 717- 725 ,(2012) , 10.1111/J.1755-0998.2012.03139.X
Juan José Robledo‐Arnuncio, Carmen Collada, Ricardo Alia, Luis Gil, Genetic structure of montane isolates of Pinus sylvestris L. in a Mediterranean refugial area Journal of Biogeography. ,vol. 32, pp. 595- 605 ,(2005) , 10.1111/J.1365-2699.2004.01196.X
E. GUICHOUX, L. LAGACHE, S. WAGNER, P. CHAUMEIL, P. LÉGER, O. LEPAIS, C. LEPOITTEVIN, T. MALAUSA, E. REVARDEL, F. SALIN, R.J. PETIT, Current trends in microsatellite genotyping. Molecular Ecology Resources. ,vol. 11, pp. 591- 611 ,(2011) , 10.1111/J.1755-0998.2011.03014.X
STEN WAHLUND, ZUSAMMENSETZUNG VON POPULATIONEN UND KORRELATIONSERSCHEINUNGEN VOM STANDPUNKT DER VERERBUNGSLEHRE AUS BETRACHTET Hereditas. ,vol. 11, pp. 65- 106 ,(2010) , 10.1111/J.1601-5223.1928.TB02483.X