Polymorphic Simple Sequence Repeats in Nuclear and Chloroplast Genomes: Applications to the Population Genetics of Trees

摘要: Molecular markers, to be used in the population genetics of forest trees, should ideally allow easy and fast genotyping, codominant, reproducible over time space, fully transferable among labs have a high information content. None available molecular marker systems meets all these requirements. We decided focus on Simple Sequence Repeats (SSRs or microsatellites) because they are reproducible, highly informative exchange even though development costs. isolated AC/GT AG/CT SSRs from Norway spruce (Picea abies K.) nuclear genome. Based hybridization data we estimate that there is one AC SSR every 500–600 kb AG 200–300 kb. Given genome size (30–40 pg) this corresponds an extremely large number for analysis. several hundreds positive clones small-insert genomic library following sequence analysis designed primers 36 them, 24 containing 12 SSRs. Twenty-two percent primer pairs produced single-locus hypervariable pattern, with remaining ones giving either single monomorphic product (22%) very poor amplification (19%) multiple bands (37%). Mendelian segregation was demonstrated loci amplified simple variable pattern. screened panel 18 trees at loci. The average alleles per locus 14 expected heterozygosity 0.80, up 23 heterozygosities exceeding 0.94. This shows can useful markers conifers overall efficiency identification process quite low due complexity