Optimising the biocompatibility of 3D printed photopolymer constructs in vitro and in vivo.

摘要: 3D printing is a rapid and accessible fabrication technology that engenders creative custom design solutions for cell scaffolds, perfusion systems culture tissue engineering. Critical to its success the biocompatibility of materials used, which should allow long-term without affecting viability or inducing an inflammatory response in vitro vivo applications. Polyjet printers offer arguably highest resolution with fewest constraints any commercially available systems. Although widely used rapid-prototyping medical devices anatomical modelling, polyjet has not been adopted by engineering field, largely due cytotoxicity leachates from printed parts. Biocompatibility context commonly addressed materials, as they tend be optimised their ability fabricate complex structures. In order study potential issues surrounding leaching toxins, we prepared substrates using MED610 photopolymer. The were cleaned either manufacturer-specified 'biocompatible' washing procedures, novel protocol incorporating sonication isopropanol water step. We then compared effectiveness these both vivo. Using primary mouse myoblast cultures, manufacturer's led inconsistent poorer when (p = 0.0002 at 48 h after indirect exposure). Subdermal implantation into nude rats demonstrated significant foreign body greater number giant cells 0.0161) bodies 0.0368) protocol, was comparable control (sham) groups. These results present improved, cytocompatible cleaning printable photopolymers facilitate 3D-printed designs