Epstein-Barr virus-associated gastric carcinoma and Epstein-Barr virus infection of the stomach.

摘要: Background Epstein-Barr virus (EBV) has been found to be associated with a type of gastric carcinoma (EBVaGC). However, many questions remain unanswered, such as epidemiology, and pathologic features EBVaGC the significance EBV in genesis EBVaGC. Experimental design Gastric non-neoplastic mucosa were evaluated reveal following issues: incidence Japanese population, genotype, clonality, gene-expression EBVaGC, localization stomach, serum titer anti-EBV antibodies EBVaGC-carrying patients. Results Using PCR EBER1 situ hybridization, (definitely amplifiable EBV-DNA positive EBER1-signal nuclei cells) was 8 72 carcinomas (11%). The dominant genotype A (7/8), C (6/8), F (8/8) restriction enzyme polymorphism, which are predominant throat washing general population Japan. cardia (4/8) or body consisted 7 advanced 1 intramucosal carcinoma. By Southern blot analysis hybridized right- left-side probe adjacent terminal repeats, present monoclonal episomal form all lacked expression EBNA2 (0/8) LMP1 by immunocytochemistry. In mucosa, signal identified infiltrating lymphocytes shedding epithelial cells predominantly fundic gland patients (8/8). Patients showed high titers anti-VCA IgG (8/8), IgA (2/8) anti-EA (7/8) just before surgery. Conclusions may infect surface epithelium stomach through reactivated EBV-carrying lymphocytes. factor initiating Anti-EBV hybridization help identify at risk for