Protein changes in the human lens during development of sentile nuclear cataract
作者： Hans A. Kramps , Herman J. Hoenders , J. Wollensak
DOI: 10.1016/0005-2795(76)90032-5
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摘要: Abstract Senile nuclear cataractous lenses were divided into three groups of increasing color. These considered as successive stages in the development senile cataract. The cortex and nucleus normal separated water-soluble, urea-soluble urea-insoluble fractions. Fractionation on a Sephadex G-200 column water-soluble components revealed five protein fractions for both nucleus. Only minor quantitative differences polypeptide chain composition found by isoelectric focusing between corresponding isolated from lenses. weight percentages determined. A decrease amounts concomitant increase fraction observed function cataract development. Lens wet content did not change significantly. carbohydrate increased, that decreased. striking phospholipid was found.
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Abraham Spector, Lu-Ku Li, Robert C. Augusteyn, Arthur Schneider, Thomas Freund, α-Crystallin. The isolation and characterization of distinct macromolecular fractions Biochemical Journal. ,vol. 124, pp. 337- 343 ,(1971) , 10.1042/BJ1240337
Frans S. M. KLEEF, Monique J. C. M. NIJZINK-MAAS, Herman J. HOENDERS, Intracellular Degradation of α-Crystallin FEBS Journal. ,vol. 48, pp. 563- 570 ,(1974) , 10.1111/J.1432-1033.1974.TB03798.X
J. P. Koopman, J. P. Van Oeveren, F. G. J. Janssen, Use of Combusted Natural Gas to Cultivate the Anaerobic Bacterial Flora from the Cecum Contents of Mice Applied Microbiology. ,vol. 26, pp. 584- 588 ,(1973) , 10.1128/AM.26.4.584-588.1973
R.M. Broekhuyse, Phospholipids in tissues of the eye I. Isolation, characterization and quantitative analysis by two-dimensional thin-layer chromatography of diacyl and vinyl-ether phospholipids Biochimica et Biophysica Acta (BBA) - Lipids and Lipid Metabolism. ,vol. 152, pp. 307- 315 ,(1968) , 10.1016/0005-2760(68)90038-6
G. Maraini, R. Mangili, Characteristics of alpha-crystallin in human senile cataract Experimental Eye Research. ,vol. 16, pp. 123- 129 ,(1973) , 10.1016/0014-4835(73)90307-2
Michel. DuBois, K. A. Gilles, J. K. Hamilton, P. A. Rebers, Fred. Smith, Colorimetric Method for Determination of Sugars and Related Substances Analytical Chemistry. ,vol. 28, pp. 350- 356 ,(1956) , 10.1021/AC60111A017
Gerard J. van Kamp, Herman J. Hoenders, The distribution of the soluble proteins in the calf lens Experimental Eye Research. ,vol. 17, pp. 417- 426 ,(1973) , 10.1016/0014-4835(73)90221-2
G. Auricchio, M. Testa, Some Biochemical Differences Between Cortical (Pale) and Nuclear (Brown) Cataracts Ophthalmologica. ,vol. 164, pp. 228- 235 ,(1972) , 10.1159/000306755
Sidney Lerman, Characterization of the insoluble protein fraction in the ocular lens Canadian Journal of Biochemistry. ,vol. 47, pp. 1115- 1119 ,(1969) , 10.1139/O69-179
A.F. van Dam, Purification and composition studies of βs-crystallin Experimental Eye Research. ,vol. 5, pp. 255- IN9 ,(1966) , 10.1016/S0014-4835(66)80035-0