Immunonephelometric Quantification of Free Haemoglobin
作者： M. Lammers , A. M. Gressner
DOI: 10.1515/CCLM.1987.25.6.363
关键词:
摘要: A fully mechanized immunonephelometric method for rapid and specific determination of free haemoglobin in serum, plasma, or aqueous haemolysates is described. It utilizes commercially available rabbit antiserum against human the Behring Nephelometer Analyser. single performed within 6 minutes requires 250 microliters total sample volume. The measuring range about 9-2300 mg/ml haemoglobin. Precision characterized by intraassay coefficients variation 3.4% 1.6%, interassay 8.2% 5.6% concentrations 29 mg/l 119 mg/l, respectively. Accuracy shown close correlation to cyanohaemiglobin spectrometry (r = 0.9837). Interference may occur visibly lipaemic samples. preliminary comparison different specimens drawn parallel shows lowest citrate followed heparin plasma while EDTA-plasma unsuitable this analysis.
hu-berlin.de
core.ac.uk参考文章(18)
Peter T. Rowley, Richard A. Doherty, Cheryl Rosecrans, Elsa Cernichiari, Sickle hemoglobin: a specific radioimmunoassay. Blood. ,vol. 43, pp. 607- 611 ,(1974) , 10.1182/BLOOD.V43.4.607.607
Jerome B. Simon, Occult blood screening for colorectal carcinoma: A critical review Gastroenterology. ,vol. 88, pp. 820- 837 ,(1985) , 10.1016/0016-5085(85)90158-1
G Virella, J Munoz, M F Lopes-Virella, B Ward, R Gadsden, Rapid immunochemical determination of free hemoglobin in plasma and urine with a laser nephelometer. Clinical Chemistry. ,vol. 25, pp. 497- 499 ,(1979) , 10.1093/CLINCHEM/25.3.497
Norbert W. Tietz, Fundamentals of Clinical Chemistry ,(1970)
M H Zweig, A Jackson, Ascorbic acid interference in reagent-strip reactions for assay of urinary glucose and hemoglobin Clinical Chemistry. ,vol. 32, pp. 674- 677 ,(1986) , 10.1093/CLINCHEM/32.4.674
S S Levinson, J Goldman, Measuring hemoglobin in plasma by reaction with tetramethylbenzidine. Clinical Chemistry. ,vol. 28, pp. 471- 474 ,(1982) , 10.1093/CLINCHEM/28.3.471
R Engler, J Pointis, Y Rondeau, C Judon, M Waks, Determination immunochimique de l'hemoglobine humaine dans les liquides biologiques Clinica Chimica Acta. ,vol. 77, pp. 159- 165 ,(1977) , 10.1016/0009-8981(77)90023-7
M J Turunen, K Liewendahl, P Partanen, H Adlercreutz, Immunological detection of faecal occult blood in colorectal cancer British Journal of Cancer. ,vol. 49, pp. 141- 148 ,(1984) , 10.1038/BJC.1984.26
Jamshid Javid, Penelope K. Pettis, Ronald J. Koenig, Anthony Cerami, Immunologic Characterization and Quantification of Haemoglobin A1c British Journal of Haematology. ,vol. 38, pp. 329- 337 ,(1978) , 10.1111/J.1365-2141.1978.TB01051.X
F. Garver, M. Baker, C. Jones, M Gravely, G Altay, T. Huisman, Radioimmunoassay for abnormal hemoglobins Science. ,vol. 196, pp. 1334- 1336 ,(1977) , 10.1126/SCIENCE.867032