An assemblage of Frankia Cluster II strains from California contains the canonical nod genes and also the sulfotransferase gene nodH

摘要: The ability to establish root nodule symbioses is restricted four different plant orders. Soil actinobacteria of the genus Frankia can a symbiotic relationship with diverse group plants within eight families from three orders, Cucurbitales, Fagales and Rosales. Phylogenetically, strains be divided into clusters, which (I, II, III) contain strains. Members Cluster II nodulate broadest range host species two growing on six continents. Two genomes were sequenced thus far, both Asia. In this paper we present first cluster genome North America (California), Dg2, represents metagenome major one minor A phylogenetic analysis core 16 shows that ancestral in genus, also non-symbiotic IV. Dg2 contains canonical nod genes nodABC for production lipochitooligosaccharide Nod factors, but copies sulfotransferase gene nodH. rhizobial systems, sulfation factors affects their specificity stability. comparison region previously Dg1 strain Pakistan common ancestor should have contained NodH proteins are sister proteins. glnA-based all sampled far supports hypothesis came Datisca glomerata following Madrean-Tethyan pattern.