Cytoprotective Activities of Milk Thistle Seed Oil Used in Traditional Tunisian Medicine on 7-Ketocholesterol and 24S-Hydroxycholesterol-Induced Toxicity on 158N Murine Oligodendrocytes

摘要: The Asteraceae family is economically very important, because many of these plants are grown mainly for their food value, such as lettuce (Lactuca), chicory (Cichorium), and sunflower (Heliantus aminus). One the typical properties this family, which includes milk thistle (Sylibum marianum), richness oil in various compounds (flavonoids, alkaloids, tocopherols, unsaturated fatty acids). Currently, coming decades, age-related diseases, including neurodegenerative a major public health problem. Preventing appearance or opposing evolution objective. In context, cytoprotective activities seed produced Tunisia were studied on 158N model using 7-ketocholesterol (7KC) 24S-hydroxycholesterol (24S) cytotoxic agents. 7KC 24S used they can be increased brain body fluids patients with Alzheimer’s Parkinson’s diseases. order to evaluate oil, complementary techniques microscopy, flow cytometry, biochemistry used. chemical composition has also been determined by chromatography techniques. Milk oils from different area rich tocopherols strongly antioxidant according biochemical tests (KRL (Kit Radicaux Libres), FRAP (Ferric Reducing Antioxidant Power), DPPH (2,2-diphenyl-1-picrylhydrazyl)). main acids linoleic acid (C18:2 n-6) oleic (C18:1 n-9). polyphenols identified homovanillic acid, p-coumaric quercetin, apigenin, predominance vanillic acid. On cells, attenuates cytotoxicity including: loss cell adhesion, plasma membrane permeability, mitochondrial dysfunction, overproduction reactive oxygen species, induction apoptosis, autophagy. attenuation observed that α-tocopherol positive control. presence nigella considered potentially cytotoxic, no effects observed. Given characteristics, properties, our results highlight potential benefit human health.