Collapsin-induced growth cone collapse mediated by an intracellular protein related to UNC-33

摘要: Collapsin, a member of the newly recognized semaphorin family, contributes to axonal pathfinding during neural development by inhibiting growth cone extension. The mechanism collapsin action is poorly understood. Here we use Xenopus laevis oocyte expression system identify molecules involved in signalling, because several experiments have raised possibility that heterotrimeric GTP-binding proteins might participate these events. A response mediator protein relative molecular mass (M(r)) 62K (CRMP-62) required for collapsin-induced inward currents X. oocytes isolated. CRMP-62 shares homology with UNC-33, nematode neuronal appropriately directed localized exclusively developing chick nervous system. Introduction anti-CRMP-62 antibodies into dorsal root ganglion neurons blocks collapse. appears be an intracellular component signalling cascade initiated unidentified transmembrane collapsin-binding protein.