Intestinal brush-border-associated enzymes: co-ordinated expression in colorectal cancer.

摘要: The brush border of normal small-intestine epithelial cells is rich in enzymes that are involved the digestive process. Such molecules can be used as markers to analyze cell lineages and differentiation properties colorectal cancers. Monoclonal antibodies detecting dipeptidyl peptidase-IV, aminopeptidase N, endopeptidase F, sucrase-isomaltase, alkaline phosphatase, maltase-glucoamylase lactase have been phenotype cancers, adjacent mucosa histologically distant mucosa. avidin-biotin peroxidase complex method was used. Expression sucrase-isomaltase phosphatase common non-neoplastic to, from, tumor; contrast, were rarely expressed more frequently tumor. Dipeptidyl whereas expressed. Two general patterns antibody reactivity observed: diffuse cytoplasmic apical; apical generally associated with differentiated tumors. A logistic predictive regression model indicated enzyme expression cancers followed a coordinate pattern, but unrelated location tumor, Dukes stage or grade. In conclusion, brush-border-associated occurs regulated co-ordinated manner. These for phenotypic sub-classification