作者: Ming-Zhu Ding , Hua Lu , Jing-Sheng Cheng , Yao Chen , Jing Jiang
DOI: 10.1007/S12010-012-9852-Z
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摘要: Comparative metabolomics was carried out to investigate the metabolic differences of Penicillium chrysogenum in pilot and industrial fermentations that resulted from scale-up. By principal component analysis, early stages two fermentation processes were clearly distinguished, whereas middle final clustered together. It indicated different metabolisms cells mainly existed during stage. Furthermore, levels polyamines, polyols, glycolysis, tricarboxylic acid cycle intermediates, which changed more dramatically process, all higher than This conditions stage should be focus process management is aimed at increasing penicillin production. Additionally, comparative accumulations precursors (valine, cysteine, lysine) revealed biosynthesis affected fermentation. These findings provide new insights further regulate improve production penicillin. More generally, this study attempts address scarcity studies contrast outcomes between commercial- pilot-scale conditions.