Comparative metabolomic study of Penicillium chrysogenum during pilot and industrial penicillin fermentations.

作者: Ming-Zhu Ding , Hua Lu , Jing-Sheng Cheng , Yao Chen , Jing Jiang

DOI: 10.1007/S12010-012-9852-Z

关键词:

摘要: Comparative metabolomics was carried out to investigate the metabolic differences of Penicillium chrysogenum in pilot and industrial fermentations that resulted from scale-up. By principal component analysis, early stages two fermentation processes were clearly distinguished, whereas middle final clustered together. It indicated different metabolisms cells mainly existed during stage. Furthermore, levels polyamines, polyols, glycolysis, tricarboxylic acid cycle intermediates, which changed more dramatically process, all higher than This conditions stage should be focus process management is aimed at increasing penicillin production. Additionally, comparative accumulations precursors (valine, cysteine, lysine) revealed biosynthesis affected fermentation. These findings provide new insights further regulate improve production penicillin. More generally, this study attempts address scarcity studies contrast outcomes between commercial- pilot-scale conditions.

参考文章(43)
D.H. Jennings, Polyol Metabolism in Fungi Advances in Microbial Physiology Volume 25. ,vol. 25, pp. 149- 193 ,(1985) , 10.1016/S0065-2911(08)60292-1
Nina Gunnarsson, Anna Eliasson, Jens Nielsen, Control of fluxes towards antibiotics and the role of primary metabolism in production of antibiotics. Advances in Biochemical Engineering \/ Biotechnology. ,vol. 88, pp. 137- 178 ,(2004) , 10.1007/B99260
W. A. Taber, G. Tertzakian, Sequential Primary and Secondary Shunt Metabolism in Penicillium chrysogenum1 Applied Microbiology. ,vol. 13, pp. 590- 594 ,(1965) , 10.1128/AM.13.4.590-594.1965
Oliver Fiehn, Joachim Kopka, Peter Dörmann, Thomas Altmann, Richard N. Trethewey, Lothar Willmitzer, Metabolite profiling for plant functional genomics Nature Biotechnology. ,vol. 18, pp. 1157- 1161 ,(2000) , 10.1038/81137
Jens Nielsen, Preben Krabben, Hyphal growth and fragmentation of Penicillium chrysogenum in submerged cultures Biotechnology and Bioengineering. ,vol. 46, pp. 588- 598 ,(1995) , 10.1002/BIT.260460612
Ming-Zhu Ding, Jing-Sheng Cheng, Wen-Hai Xiao, Bin Qiao, Ying-Jin Yuan, Comparative metabolomic analysis on industrial continuous and batch ethanol fermentation processes by GC-TOF-MS Metabolomics. ,vol. 5, pp. 229- 238 ,(2009) , 10.1007/S11306-008-0145-Z