Protein secondary-shell interactions enhance the photoinduced hydrogen production of cobalt protoporphyrin IX

摘要: Hydrogen is an attractive fuel with potential for production scalability, provided that inexpensive, efficient molecular catalysts utilizing base metals can be developed hydrogen production. Here we show the first time cobalt myoglobin (CoMyo) catalyzes in mild aerobic conditions turnover number of 520 over 8 hours. Compared to free Co-protoporphyrin IX, incorporation into scaffold results a 4-fold increase photoinduced activity. Engineered variants which specific histidine resides proximity active site were mutated alanine result modulation catalytic activity, H64A/H97A mutant displaying activity 2.5-fold higher than wild type. Our demonstrate protein scaffolds augment and modulate intrinsic catalysts.