Cytoplasmic factors do not contribute to a maternal effect on ethanol teratogenesis.

摘要: Both maternal and fetal genetic factors influence variations in response to prenatal ethanol exposure. To assess the effect of genotype on incidence teratogenesis, a reciprocal cross study was conducted an animal mode using relatively susceptible C57BL/6J (B6) resistant DBA/2J (D2) inbred mice. This mating pattern produced four embryonic genotypes: true-bred B6B6 D2D2 litters hybrid B6D2 D2B6 litters. examine role egg cytoplasm as source variation that could account for effect, F1 females were mated back B6 males, which two additional B6D2.B6 D2B6.B6. Dams intubated with either 5.8 g/kg or isocaloric amount maltose-dextrin day 9 pregnancy. On 18 pregnancy, dams sacrificed, fetuses removed, weighed, sexed, examined gross morphological malformations. Every other fetus within litter prepared skeletal soft tissue analysis. Results showed higher rate teratogenesis group compared genetically similar group, indicates susceptibility teratogenesis. The percentage affected male female did not differ, suggests sex-linked are responsible effect. backcross D2B6.B6 differ significantly any measure suggesting differences maternally transmitted cytoplasmic material cause Factors uterine environment genomic imprinting. Separating from fetal-mediated mechanisms is needed identifying mothers infants at risk.