Embryo transfers between C57BL/6J and DBA/2J mice: Examination of a maternal effect on ethanol teratogenesis.

摘要: Genetic factors influence Fetal Alcohol Spectrum Disorders (FASD) in both humans and animals. Experiments using inbred selectively bred mouse stocks that controlled for 1) ethanol dose, 2) maternal fetal blood levels, 3) developmental exposure stage, show genotype can affect teratogenic outcome. Other experiments distinguish the effects mediated by from those genotype. One technique to versus effect is utilize embryo transfers. This study first examine teratogenesis - weight deficits mortality, digit, kidney, vertebral malformations C57BL/6J (B6) DBA/2J (D2) fetuses were transferred as blastocysts into B6 D2 dams. We hypothesized that, following alcohol exposure, gestating within mothers, compared will exhibit a higher frequency of malformations. On day 9 pregnancy, females intubated (IG) with either 5.8 g/kg (E) or maltose dextrin (MD). mated strain treated maltose, not exposed treatment. Implantation rates affected Results more embryos implanted than (p<.05; 47% vs 23%, respectively). There was no difference percentage implanting (14% 16%, respectfully). Litter mortality averaged 24% across all experimental groups. Overall, utero reduced mean litter treatment (E=1.01 g; MD=1.19 p<.05); but litters weighed similarly natural (1.30 g 1.11 p<.05). Approximately 50% exhibited some malformation (digit, vertebral, and/or kidney) regardless whether they female, naturally conceive