Molecular epidemiology of Cryptococcus neoformans and Cryptococcus gattii in China between 2007 and 2013 using multilocus sequence typing and the DiversiLab system.

摘要: The purpose of this study was to investigate the molecular characteristics 83 clinical Cryptococcus neoformans/C. gattii species complex isolated in Beijing, China, between 2007 and 2013. Restriction fragment length polymorphism gene URA5 (URA5-RFLP), multilocus sequence typing (MLST), automated repetitive polymerase chain reaction (rep-PCR; DiversiLab system) were performed genotype these cryptococcal isolates. There an excellent correlation amongst three methods; however, PU157 assigned as VNII according URA5-RFLP, while it classified VNI by system analysis. finally identified VNB seven-locus MLST Moreover, though AD hybrids could not be processed MLST, ideal results obtained system. accounted for 95.2 % (79/83) Besides one strain VNB, VNIII, VGI each, a VGII detected our study, which from patient temperate region North China. In addition, most common type (ST) ST5, accounting 91.6 % (76/83), followed ST31, ST63, ST182, ST295, ST296, ST332. ST332 new STs. Except isolate (VNB), identical quickly accurately through compared URA5-RFLP. discovery climate regions China suggested that population structure C. neoformans should explored more extensively. Our also showed can used genotyping gattii.