Relative quantitative proteomic analysis reveals wound response proteins correlated with after‐cooking darkening

摘要: Many common potato tuber defects are difficult to elicidate because of the degree genetic complexity involved, making systems biology approaches necessary. Interaction between chlorogenic acid and iron is responsible for darkening tissues upon heating – termed after-cooking (ACD). To explore mechanisms severity in tissues, we have employed relative quantitative proteomics discover differentially expressed proteins involved ACD. Tuber tissue samples were collected from a family diploid clones which possess highly segregated darkening. Exploiting this segregation, as well observation that more prevalent stem end than apical end, three sample groups formed: (i) ends high-ACD clones, (ii) low-ACD (iii) clones. Protein digested labeled using isotopic reductive methylation, allowing an orthogonal two-way comparison protein profiles 2-D-LC-MS/MS. Using cutoff fold change 2 high- groups, 30 showed correlation with Overall, observed changes abundance enhanced wound-response program tissues. Among these proteins, five further validated at transcript level qRT-PCR. These may be incorporated into design strategies create cultivars low levels