A general method for identifying recessive diploid-specific mutations in Saccharomyces cerevisiae, its application to the isolation of mutants blocked at intermediate stages of meiotic prophase and characterization of a new gene SAE2.

摘要: We describe a general new approach for identifying recessive mutations that affect diploid strains of yeast Saccharomyces cerevisiae and the application this method to identification confer an intermediate block in meiotic prophase chromosome metabolism. The uses temperature-sensitive conjugation mutation ste7-1 combination with homothallism. interest defect spore formation is dependent upon gene required initiation recombination development meiosis-specific structure (SPO11). Identified screen were null DMC1 gene, nonnull RAD50 (rad50S), three genes designed SAE1, SAE2 SAE3 (Sporulation Absence Spo Eleven). Molecular characterization mitotic phenotypes sae2 mutants are also presented. conferred by virtually indistinguishable from those previously identified (rad50S). Most notably, both only weak sensitivity radiomimetic agent methyl methane sulfonate (MMS) but completely resection turnover double-strand breaks. These observations provide further evidence constellation identifies specific molecular function.