Combination of single- and double-stranded conformational polymorphism for direct discrimination of gastric Helicobacter pylori
作者： Chuancang Jiang , Chuanfu Li , David S Chi , Donald A Ferguson , Tuanzhu Ha
DOI: 10.1016/S0167-7012(98)00058-X
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摘要: Abstract Molecular typing of strains among the highly diverse population Helicobacter pylori ( H. ) is an important approach for both basic and clinical studies. Genomic DNAs prepared from 18 gastric biopsy specimens, 21 isolates obtained five collected a single patient at weekly intervals, were subjected to combined single- double-stranded conformational polymorphism (SDSCP) assay. The results showed that 19 tested discriminated by SDSCP analysis. analysis same different times resulted in identical profiles, suggesting reproducibility method. When DNA preparations specimens analysis, unique profiles generated matched those their corresponding cultured each patient. For comparison, polymerase chain reaction (PCR)-based restriction fragment length yielded only nine 20 strains. data suggest may be effective reliable method differentiation directly without requiring isolation organisms culture.
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