An efficient method for selecting unique-sequence clones from DNA libraries and its application to fluorescent staining of human chromosome 21 using in situ hybridization
作者： James C. Fuscoe , Colin C. Collins , Dan Pinkel , Joe W. Gray
DOI: 10.1016/0888-7543(89)90092-X
关键词:
摘要: This paper describes an efficient procedure for selecting large numbers of unique-sequence or very low repeat-sequence probes from recombinant phage libraries. Probes were selected the Charon 21A library LL21NS02 (made DNA human chromosome 21) in a multistep process which (1) inserts subcloned into Bluescribe plasmids, (2) plasmids grown at high density colonies on nitrocellulose, and (3) as containing if failed to hybridize, stringency, radiolabeled total DNA. In this manner, 1530 picked form pBS-U21/1530. About 80% recombinants constituting pBS-U21/1530 shown by Southern analysis carry that are present only one copy haploid genomic Approximately 70% sequences mapped 21. Fluorescence situ hybridization with allowed specific, intense staining number 21 chromosomes metaphase spreads made lymphocytes.
elsevier.com
sci-hub.se 参考文章(23)
Savio L.C. Woo, [26] A sensitive and rapid method for recombinant phage screening Methods in Enzymology. ,vol. 68, pp. 389- 395 ,(1979) , 10.1016/0076-6879(79)68028-X
James W Schumm, Robert G Knowlton, Jeffrey C Braman, David F Barker, D Botstein, G Akots, Valerie A Brown, Thomas C Gravius, C Helms, Kathy Hsiao, Kenneth Rediker, Joan G Thurston, Helen Donis-Keller, Identification of more than 500 RFLPs by screening random genomic clones. American Journal of Human Genetics. ,vol. 42, pp. 143- 159 ,(1988)
C A Weber, E P Salazar, S A Stewart, L H Thompson, Molecular cloning and biological characterization of a human gene, ERCC2, that corrects the nucleotide excision repair defect in CHO UV5 cells. Molecular and Cellular Biology. ,vol. 8, pp. 1137- 1146 ,(1988) , 10.1128/MCB.8.3.1137
Richard A. Kramer, John R. Cameron, Ronald W. Davis, Isolation of bacteriophage λ containing yeast ribosomal RNA genes: Screening by in situ RNA hybridization to plaques Cell. ,vol. 8, pp. 227- 232 ,(1976) , 10.1016/0092-8674(76)90006-4
James C. Fuscoe, Human chromosome-specific DNA libraries: use of an oligodeoxynucleotide probe to detect nonrecombinants Gene. ,vol. 52, pp. 291- 296 ,(1987) , 10.1016/0378-1119(87)90056-4
J. Perlman, J.C. Fuscoe, Molecular characterization of the purity of seven human chromosome-specific DNA libraries. Cytogenetic and Genome Research. ,vol. 43, pp. 87- 96 ,(1986) , 10.1159/000132302
M. Dagert, S.D. Ehrlich, Prolonged incubation in calcium chloride improves the competence of Escherichia coli cells. Gene. ,vol. 6, pp. 23- 28 ,(1979) , 10.1016/0378-1119(79)90082-9
W. Benton, R. Davis, Screening lambdagt recombinant clones by hybridization to single plaques in situ Science. ,vol. 196, pp. 180- 182 ,(1977) , 10.1126/SCIENCE.322279
A. R. Wyman, R. White, A highly polymorphic locus in human DNA. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 77, pp. 6754- 6758 ,(1980) , 10.1073/PNAS.77.11.6754
Andrew P. Feinberg, Bert Vogelstein, A technique for radiolabeling DNA restriction endonuclease fragments to high specific activity Analytical Biochemistry. ,vol. 132, pp. 6- 13 ,(1983) , 10.1016/0003-2697(83)90418-9