Isolation and Characterization of Hepatic Cancer Cells with Stem-Like Properties from Hepatocellular Carcinoma

摘要: Background & aims Major burdens in the treatment of hepatocellular carcinoma (HCC) are high percentage recurrence and resistance to chemotherapy. Hepatic cancer stem cells provide a reservoir that can self-renew, maintain tumor by generating differentiated non-stem which make up bulk responsible for after ablative surgery chemoradiotherapy. The objective this study was identify characterize self-renewing subpopulation human liver with distinctive genetic profile adds capacity proliferate despite chemotherapy promotes recurrence. Methods Stemness properties isolated from HCC biopsy were established their form spheroids cell proliferation assays. also showed enhanced chemoresistance drugs. up-regulation markers is proven immunocytochemistry stainings reverse transcription-PCR. Results Cells had proliferative potential, even when cultured medium supplemented doxorubicin carboplatin, eliminated Rhodamine 123 immediately culture formed suspension. Molecular diagnosis techniques expressed Oct 3/4 CXCR4. positive CD133 CD90 specific markers, monoclonal antibody staining. Conclusion unique characteristics identified explain self-renewal could drive metastasis patients have received cancer. identification cloning such aid developing better therapeutic approaches HCC, as chemosensitive pretherapeutic assays or targeted therapies.