Molecular surveillance of anti-malarial resistance Pfdhfr and Pfdhps polymorphisms in African and Southeast Asia Plasmodium falciparum imported parasites to Wuhan, China.

摘要: Anti-malarial drug resistance is a severe challenge for eventual control and global elimination of malaria. Resistance to sulfadoxine-pyrimethamine (SP) increases as mutations accumulate in the Pfdhfr Pfdhps genes. This study aimed assess polymorphisms prevalence mutation these genes Plasmodium falciparum infecting migrant workers returning Wuhan, China. Blood samples were collected 9 years (2011–2019). Parasite genomic DNA was extracted from blood spots on filter paper. The evaluated by nested PCR sequencing. single-nucleotide (SNPs) haplotypes analysed. codon 108 showed 94.7% rate, while Pfdhps, rate 437 79.0%. In total, five unique at locus 11 found Pfdhfr-Pfdhps combined loci revealed 28 haplotypes. A triple mutant (IRNI) most prevalent haplotype (84.4%). For single (SGKAA) double (SGEAA) detected frequencies 37.8 22.3%, respectively. Among haplotypes, quadruple (IRNI-SGKAA) common, with 30.0% frequency, followed quintuplet (IRNI-SGEAA) frequency 20.4%. high saturation medium demonstrated present data will provide support predicting status progression antifolate malaria-endemic regions imported malaria nonendemic areas. Additional interventions evaluate prevent SP should be continuously considered.