Rolling circle amplification-based detection of human topoisomerase I activity on magnetic beads.
作者： Laura Zuccaro , Cinzia Tesauro , Barbara Cerroni , Alessio Ottaviani , Birgitta Ruth Knudsen
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摘要: A high-sensitivity assay has been developed for the detection of human topoisomerase I with single molecule resolution. The method uses magnetic sepharose beads to concentrate rolling circle products, produced by amplification DNA molecules circularized and detectable a confocal microscope as discrete dots, once reacted fluorescent probes. Each dot, corresponding cleavage–religation event mediated enzyme, can be counted due its high signal/noise ratio, allowing 0.3 pM enzyme representing valid detect activity in highly diluted samples.
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sci-hub.se 参考文章(19)
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