Immunohistochemical p53 expression patterns in sarcomatoid carcinomas of the upper respiratory tract.

摘要: Sarcomatoid carcinoma of the upper respiratory tract is a phenotypically complex neoplasm that has triggered much thoughtful discussion regarding histogenic origin and morphologic classification. In particular, its putative epithelial lineage distinction from pseudosarcomatous reaction are sometimes questioned. Little known about genetic alterations underlying sarcomatoid carcinoma. Although 45% conventional squamous cell carcinomas harbor p53 mutations, status not well established. immunohistochemical analysis using monoclonal antibody D07 was performed on 23 tract. Twenty tumors were biphasic, having dual spindled components. four these biphasic tumors, components separately analyzed for gene mutations by sequence analysis. immunohistochemistry also 19 cases postradiation stromal atypia. Strong diffuse staining detected in 18 (78%) carcinomas. When component compared with corresponding component, identical patterns protein expression noted (95%) 20 tumors. Weak observed one (5%) evaluated DNA analysis, always same paired spindle These findings help further dispel notion represents reactive proliferation (pseudosarcoma) or collision between sarcoma (collision tumor). Instead, share common pathway tumorigenesis despite their conspicuous divergence at phenotypic level.