Metabolites Identified during Varied Doses of Aspergillus Species in Zea mays Grains, and Their Correlation with Aflatoxin Levels

摘要: Aflatoxin contamination is associated with the development of aflatoxigenic fungi such as Aspergillus flavus and A. parasiticus on food grains. This study was aimed at investigating metabolites produced during fungal maize their correlation aflatoxin levels. Maize cobs were harvested R3 (milk), R4 (dough), R5 (dent) stages maturity. Individual kernels inoculated in petri dishes four doses spores. Fungal colonisation, metabolite profile, levels examined. Grain colonisation decreased kernel maturity: milk-, dough-, dent-stage by approximately 100%, 60%, 30% respectively. increased dose dough dent stages. Polar including alanine, proline, serine, valine, inositol, iso-leucine, sucrose, fructose, trehalose, turanose, mannitol, glycerol, arabitol, myo-inositol, some intermediates tricarboxylic acid cycle (TCA—also known citric or Krebs cycle) important for classification. Important non-polar included arachidic, palmitic, stearic, 3,4-xylylic, margaric acids. correlated several polar metabolites. The strongest positive negative correlations arabitol (R = 0.48) turanose −0.53), Several interconnected TCA; interconnections TCA varied depending upon grain