Immunological screening and characterization of highly specific monoclonal antibodies against 20 kDa hGH
作者： Hongwu Du , Guangyu Chen , Shuang Wang , Shan Wang , Shaoxu Li
DOI: 10.4155/BIO.12.188
关键词:
摘要: Background: hGH has been widely abused as a doping agent in sports for many years. There are some important approaches the detection of doping, and ratio 22:20 kDa GH was considered one most suitable indicators abuse. Currently, effective anti-GH antibodies related reagents needed to develop method, particular, highly specific anti-20 monoclonal prerequisite. Herein we constructed expression vector 20 prepared corresponding by immunization recombinant human into mice. Positive clones that can specifically recognize were screened characterized enzyme immunoassay, Dot-ELISA surface plasmon resonance. In total, 14 cell lines out. Results: By series characterization, it found 6C8, 44H3, 12G7 33Y19 showing much higher specificity affinity hGH, P3H9 could both...
future-science.com
sci-hub.se 参考文章(32)
U.J. Lewis, J.T. Dunn, L.F. Bonewald, B.K. Seavey, W.P. Vanderlaan, A naturally occurring structural variant of human growth hormone. Journal of Biological Chemistry. ,vol. 253, pp. 2679- 2687 ,(1978) , 10.1016/S0021-9258(17)40874-X
A. Weltman, J. Y. Weltman, R. Schurrer, W. S. Evans, J. D. Veldhuis, A. D. Rogol, Endurance training amplifies the pulsatile release of growth hormone: effects of training intensity. Journal of Applied Physiology. ,vol. 72, pp. 2188- 2196 ,(1992) , 10.1152/JAPPL.1992.72.6.2188
Marianne Andersen, Per Hyltoft Petersen, Ole Blaabjerg, Jørgen Hangaard, Claus Hagen, None, Evaluation of growth hormone assays using ratio plots Clinical Chemistry. ,vol. 44, pp. 1032- 1038 ,(1998) , 10.1093/CLINCHEM/44.5.1032
Frances M. DeNoto, David D. Moore, Howard M. Goodman, Human growth hormone DNA sequence and mRNA structure: possible alternative splicing Nucleic Acids Research. ,vol. 9, pp. 3719- 3730 ,(1981) , 10.1093/NAR/9.15.3719
Zhaoqi Yan, Qiaowen Wu, Hongwu Du, Shuang Wang, Yuzhi Wei, Guangyu Chen, Xiaowei Liu, Haiming Jin, Generation and Utilization of Anti-drug Monoclonal Antibodies for Screening of 36 Drug Users by Dot-ELISA Hybridoma. ,vol. 28, pp. 145- 148 ,(2009) , 10.1089/HYB.2008.0078
SHINICHI MOMOMURA, YOSHIHIDE HASHIMOTO, YUKIO SHIMAZAKI, MINORU IRIE, Detection of exogenous growth hormone (GH) administration by monitoring ratio of 20kDa- and 22kDa-GH in serum and urine. Endocrine Journal. ,vol. 47, pp. 97- 101 ,(2000) , 10.1507/ENDOCRJ.47.97
Minoru Irie, Makoto Ueki, Yoshifumi Kishikawa, Manabu Nishii, Takashi Kawahara, 20K-GH and its use in detecting GH abuse. Growth Hormone & Igf Research. ,vol. 19, pp. 352- 356 ,(2009) , 10.1016/J.GHIR.2009.04.013
Andrew T Kicman, John P Miell, J. Derrick Teale, Jake Powrie, Peter J Wood, Paul Laidler, Peter J Milligan, David A Cowan, Serum IGF-I and IGF binding proteins 2 and 3 as potential markers of doping with human GH. Clinical Endocrinology. ,vol. 47, pp. 43- 50 ,(1997) , 10.1046/J.1365-2265.1997.2111036.X
GERHARD BAUMANN, Growth hormone heterogeneity: genes, isohormones, variants, and binding proteins. Endocrine Reviews. ,vol. 12, pp. 424- 449 ,(1991) , 10.1210/EDRV-12-4-424
Jan Frystyk, Caroline Marie Andreasen, Sanne Fisker, Determination of free growth hormone. The Journal of Clinical Endocrinology and Metabolism. ,vol. 93, pp. 3008- 3014 ,(2008) , 10.1210/JC.2008-0375