Proteomics profiling of hepatic mitochondria in heterozygous Sod2+/- mice, an animal model of discreet mitochondrial oxidative stress.

摘要: The heterozygous superoxide dismutase 2 (SOD2) gene knockout (sod2 +/- ) mouse model has been increasingly used in cardiovascular and age research, neurobiology, pharmacology/ toxicology. These mutant mice exhibit mild oxidant stress mitochondria but remain clinically inconspicuous. Although the Sod2 characterized with respect to mitochondrial function transcript expression of certain individual genes, effects singular loss allele on global hepatic proteins remains unknown. We therefore performed a differential analysis proteome from wild-type order identify consequences partial deletion. Using 2-D difference gel electrophoresis (DICE) coupled MALDI-MS/MS, we found approximately 1500 protein spots, which 57 were differentially expressed (≥1.5-fold change). Both SOD 1 downregulated, other antioxidant enzymes related upregulated (