IPF2α-I: An index of lipid peroxidation in humans

摘要: Isoprostanes are prostaglandin isomers produced from arachidonic acid by a free radical-catalyzed mechanism. Urinary excretion of 8-iso-prostaglandin F2α, an isomer the PGG/H synthase (cyclooxygenase or COX) enzyme product, F2α (PGF2α), has exhibited promise as index oxidant stress in vivo. We have developed quantitative method to measure isoprostane F2α-I, (IPF2α-I) class I (8-iso-PGF2α is IV), using gas chromatography/mass spectrometry. IPF2α-I severalfold abundant human urine 8-iso-PGF2α, with mean values 737 ± 20.6 pg/mg creatinine. Both isoprostanes formed radical-dependent manner low density lipoprotein oxidized copper vitro. However, IPF2α-I, unlike not COX-dependent platelets activated thrombin collagen Similarly, COX inhibition vivo no effect on IPF2α-I. Neither serum cellular capacity generate isoprostane, nor urinary actual generation vivo, depressed aspirin indomethacin. In contrast, both thromboxane B2 and its 11-dehydro metabolite inhibitors. Although 8-iso-PGF2α formation substantially inhibitors, compound unaffected. elevated cigarette smokers compared controls (1525 180 versus 740 40 creatinine; P < 0.01) highly correlated (r = 0.9; 0.001). novel lipid peroxidation which can be measured precision sensitivity. It F2-isoprostane radical- but manner. may minor product COX, this pathway contributes trivially, if at all, levels urine. smokers.