Development of IFN-gamma-producing CD8+ gamma delta+ T lymphocytes and IL-2-producing CD4+ alpha beta+ T lymphocytes during contact sensitivity.

摘要: This paper describes the development of Ag-specific proliferation and production IFN-gamma IL-2 during contact sensitivity (CS) to hapten picryl chloride (PCl). Lymph node cells from mice immunized with PCl proliferate produce when re-exposed specific Ag in vitro. Time course experiments showed that peak occurred at days 3 4 after immunization, a sharp decline by day 6. In contrast, peaked but persisted up 10. Proliferation displayed immune lymph were required different cell populations. fact, was due CD8+, gamma delta+ T cell, while presence CD4+, alpha beta+ cell. Furthermore, genetic (MHC) restriction, finer analysis using congenic strains indicated K molecule restricting element. confirmed blocking APC used trigger mAb. I-A-restricted, as demonstrated I-A APCs. Further purified revealed these produced an MHC (K)-restricted fashion. Injection mAb blocked subsequent vitro proliferation, well production, all three responses unaffected injection given time immunization. CS reaction it had no effect challenge. challenge reduced did not abolished CS, suggesting is important exclusively responsible for reaction.