Characterization of microbial communities removing nitrogen oxides from flue gas: the BioDeNOx process.

摘要: BioDeNOx is an integrated physicochemical and biological process for the removal of nitrogen oxides (NOx) from flue gases. In this process, gas purged through a scrubber containing solution Fe(II)EDTA 2− , which binds NOx to form Fe(II)EDTA·NO complex. Subsequently, complex reduced in bioreactor dinitrogen by microbial denitrification. oxidized Fe(III)EDTA − oxygen gas, regenerated iron reduction. study, communities both lab- pilot-scale reactors were studied using culture-dependent -independent approaches. A pure bacterial strain, KT-1, closely affiliated 16S rRNA analysis gram-positive denitrifying bacterium Bacillus azotoformans was obtained. DNA-DNA homology isolate with type strain 89%, indicating that KT-1 belongs species B. . Strain reduces N 2 ethanol, acetate, as electron donors. It does not reduce Denaturing gradient gel electrophoresis PCR-amplified gene fragments showed presence bacteria members phylum Deferribacteres Fe(III)-reducing group bacteria. Fluorescent situ hybridization oligonucleotide probes designed latter more dominant reactors.