Topical Effect of a Medically Prescribed Pediatric Antibiotic on Dental Biofilm: A Cross-Over, In Situ Study

摘要: OBJECTIVE This study aimed to investigate the possible topical effect of a broad-spectrum antibiotic on dental biofilm formed in situ absence or presence sucrose. METHODS A crossover was conducted three phases 14 days each, during which 11 volunteers wore palatal devices containing 6 enamel blocks covered with meshes allow formation. Dental were extraorally submitted 20% sucrose solution at different frequencies exposure (0, 3 and 8 times/day), suspension amoxicillin/clavulanate potassium (A/CP) placebo (P) an 8-hour time interval application regimen. On 14(th) day each phase, biofilms collected for microbiological (conventional culture) molecular (Denaturing Gradient Gel Electrophoresis--DGGE) analyses. RESULTS In (SE) 3-time daily frequency, treated A/CP showed lower total weight counts microbiota than ones P (p>0.05). presented higher Candida spp. when compared SE, especially 8-time frequency (p 0.05), regardless SE. However, DGGE profiles demonstrated large interindividual variability. CONCLUSION Both conventional culture have some differences exposed suspensions, mainly sucrose, suggests sugar-free biofilm.