Growth rate analysis and protein identification of Kappaphycus alvarezii (Rhodophyta, Gigartinales) under pH induced stress culture
作者： Mian Zi Tee , Yoong Soon Yong , Kenneth Francis Rodrigues , Wilson Thau Lym Yong
DOI: 10.1016/J.AQREP.2015.09.001
关键词:
摘要: Abstract Environmental pH is one of the factors contributing to abiotic stress which in turn influences growth and development macroalgae. This study was conducted order assess physiological changes Kappaphycus alvarezii under different conditions: pHs 6, ∼8.4 (control) 9. K. explants exhibited a difference daily rate (DGR) among treatments ( p ≤ 0.05). The highest DGR observed control culture with followed by alkaline (pH 9) acidic 6) induced cultures. Protein expression profile generated from cultures using sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) protein identification analysis matrix-assisted laser desorption/ionization time-of-flight mass spectrometer (MALDI-TOF-MS) Mascot software. Ribulose bisphosphate carboxylase (Rubisco) large chain identified be up-regulated condition during second fourth week culture. findings indicated that Rubisco can employed as biomarker for stress. Further on association between levels their underlying mechanisms conditions recommended.
core.ac.uk
ums.edu.my参考文章(35)
J. K. Patterson Edward, J. R. Bhatt, Impacts of cultivation of Kappaphycus alvarezii on coral reef environs of the Gulf of Mannar and Palk Bay, South-eastern India. Invasive alien plants: an ecological appraisal for the Indian subcontinent. pp. 89- 98 ,(2012) , 10.1079/9781845939076.0089
Kenneth Francis Rodrigues, Datin Ann Anton, Lie Chin Wei, Hoo Ting Siew, Thau, Wilson,Lym Yong, In vitro Micropropagation of Eucheuma Seaweeds ,(2011)
Patrick Sorgeloos, Patrick Lavens, Manual on the production and use of live food for aquaculture Published in <b>1996</b> in Rome by FAO. ,(1996)
Loretto Contreras-Porcia, Camilo Lpez-Cristoffanini, Proteomics in Seaweeds: Ecological Interpretations InTech. ,(2012) , 10.5772/37965
Christopher E. Cornwall, Christopher D. Hepburn, Daniel Pritchard, Kim I. Currie, Christina M. McGraw, Keith A. Hunter, Catriona L. Hurd, CARBON-USE STRATEGIES IN MACROALGAE: DIFFERENTIAL RESPONSES TO LOWERED PH AND IMPLICATIONS FOR OCEAN ACIDIFICATION(1). Journal of Phycology. ,vol. 48, pp. 137- 144 ,(2012) , 10.1111/J.1529-8817.2011.01085.X
Nair S. Yokoya, Yocie Yoneshigue-Valentin, Micropropagation as a tool for sustainable utilization and conservation of populations of Rhodophyta Revista Brasileira De Farmacognosia-brazilian Journal of Pharmacognosy. ,vol. 21, pp. 334- 339 ,(2011) , 10.1590/S0102-695X2011005000077
Gert Lubec, Leila Afjehi-Sadat, Limitations and pitfalls in protein identification by mass spectrometry Chemical Reviews. ,vol. 107, pp. 3568- 3584 ,(2007) , 10.1021/CR068213F
Yoong Soon Yong, Wilson Thau Lym Yong, Ann Anton, Analysis of formulae for determination of seaweed growth rate Journal of Applied Phycology. ,vol. 25, pp. 1831- 1834 ,(2013) , 10.1007/S10811-013-0022-7
Pooi-Fong Wong, Li-Jing Tan, Hannah Nawi, Sazaly AbuBakar, PROTEOMICS OF THE RED ALGA, GRACILARIA CHANGII (GRACILARIALES, RHODOPHYTA)1 Journal of Phycology. ,vol. 42, pp. 113- 120 ,(2006) , 10.1111/J.1529-8817.2006.00182.X
C. R. K. Reddy, Bhavanath Jha, Yuji Fujita, Masao Ohno, Seaweed micropropagation techniques and their potentials: an overview Journal of Applied Phycology. ,vol. 20, pp. 609- 617 ,(2008) , 10.1007/S10811-007-9205-4