Osteopontin and Its Receptor αvβ3 Integrin Are Coexpressed in the Human Endometrium during the Menstrual Cycle But Regulated Differentially

摘要: Osteopontin is an arginine-glycine-aspartic acid-containing acidic glycoprotein component of the extracellular matrix that postulated to bind integrin receptors at cell surface mediate cellular adhesion and migration during embryo implantation. The primary aim this study was examine uterine expression osteopontin throughout menstrual cycle in normal fertile controls sampled prospectively based on urinary LH surge detection. Expression documented using Northern blot analysis, situ hybridization, immunohistochemistry. Furthermore, temporal pattern compared with its receptor, αvβ3 integrin. Using Ishikawa cells, a well differentiated endometrial adenocarcinoma line, vitro regulation receptor studied. By mRNA appears early secretory phase, maximal occurring mid late secretory-phase endomet...