Tetraspanins CD9 and CD81 are molecular partners of trimeric FcɛRI on human antigen‐presenting cells

摘要: To cite this article: Peng WM, Yu CF, Kolanus W, Mazzocca A, Bieber T, Kraft S, Novak N. Tetraspanins CD9 and CD81 are molecular partners of trimeric FcɛRI on human antigen-presenting cells. Allergy 2011; 66: 605–611. Abstract Background:  Most functions tetraspanins not related to cell-surface receptor ligand binding, but mediated by direct interactions with their partner proteins. Functions FcɛRI, expressed cells (APCs), range from amplification allergic inflammatory reactions active suppression. Cell-type-specific protein–protein might play a role in the regulation these bidirectional tasks. Therefore, we intended study tetraspanins. Methods:  The expression levels CD9, CD37, CD53, CD63, CD81, CD82, CD151 skin dendritic atopic dermatitis (AD) patients or healthy individuals were detected flow cytometry. Tetraspanin FcɛRIpos FcɛRIneg monocyte subpopulations was evaluated. Flow cytometry, confocal microscopy, immunoprecipitation, immunoblotting experiments performed observe relationship between FcɛRI. Furthermore, plate stimulation performed, cytokines supernatants detected. Results:  We found that APCs high amounts associated Concomitant activation monocytes increased FcɛRI-mediated cytokine release. Conclusion:  Taken together, show for first time act as APC, which be importance diseases such AD.