Glutathione-Induced Calcium Shifts in Chick Retinal Glial Cells.

摘要: Neuroglia interactions are essential for the nervous system and in retina Muller cells interact with most of neurons a symbiotic manner. Glutathione (GSH) is low-molecular weight compound that undertakes major antioxidant roles glia, however, whether this could act as signaling molecule and/or glia currently unknown. Here we used embryonic avian to obtain mixed retinal or purified culture evaluate calcium shifts induced by GSH. A dose response curve (0.1-10 mM) showed 5-10 mM GSH, exclusively glial (later labeled identified 2M6 positive cells), while responded 50 KCl (labeled βIII tubulin cells). BBG 100 nM, P2X7 blocker, inhibited effects GSH on glia. However, addition DNQX 70 μM MK-801 20 μM, non-NMDA NMDA blockers, had no effect shift. Oxidized glutathione (GSSG) at 5 failed induce mobilization cells, indicating structural features promote elevations cytoplasmic levels. Indeed, short pulse (60s) protects from oxidative damage after 30 min incubation 0.1% H2O2. Finally, GABA release chick retina, neuron-glia cell cultures, which were absence sodium. also propidium iodide uptake receptor dependent Our data suggest effects, millimolar range particularly regulate GABA, additional protective neuron-glial circuit.