Flt-3 ligand, in combination with bovine granulocyte-macrophage colony-stimulating factor and interleukin-4, promotes the growth of bovine bone marrow derived dendritic cells.

摘要: Culture of bone marrow precursor cells with cytokines, including granulocyte-macrophage colony-stimulating factor (GM-CSF), interleukin-4 (IL-4) and the tyrosine kinase receptor binding proteins Flt-3 ligand (Flt-3L) stem-cell (SCF), has previously been shown, in both mouse human, to result generation large numbers dendritic cells. We extend these findings bovine GM-CSF, IL-4 either Flt-3L or SCF enhanced low buoyant-density However, only addition cultured GM-CSF was shown increase number induce differentiation potent capacity stimulate allogeneic T resting CD4+ memory The effective ability associated expression major histocompatibility complex (MHC) class II molecules CD80/86 by Bovine derived appeared be exclusively myeloid origin because they expressed myeloid-related antigens CD14, MyD-1 CD11b.