Accumulation of Boron in Human Malignant Glioma Cells in vitro is Cell Type Dependent

摘要: It has been shown that human malignant glioma tumours consist of several subpopulations tumour cells. Due to heterogeneity and different degrees vascularisation cell possess varying resistance chemo- or radiation therapy. Therefore, therapy is dependent on the ability specifically target a cell. Boron neutron capture (BNCT) bimodal method, in therapy, taking advantage stable isotope boron-10 neutrons. results disintegration products depositing large amounts energy within short length, approximately one diameter. Thereby, selective irradiation may be accomplished if sufficient amount boron accumulated hence cell-associated concentration critical importance. The accumulation boron, boronophenylalanine (BPA), was investigated two fibroblast line vitro. cells were incubated at low concentrations (0-5 microg B/ml). Oil filtration then used for separation extracellular boron. Inductively coupled plasma atomic emission spectroscopy (ICP-AES) determination. Significant (P < 0.05) differences ratio (relation between concentration) lines found. Human fibroblasts, represent normal cells, showed growth-dependent uptake lower than Our findings indicate BPA concentration, incubation time should considered BNCT.