Protection From Ischemic Cell Death by the Induction of Cytoglobin

摘要: Abstract Methods to reduce β-cell loss after islet isolation and transplantation must be developed if is become a preferred treatment for diabetes. Most recent research has focused on the reduction of toxicity from immunosuppressants enhancement revascularization by growth factors such as vascular endothelial factor. Cytoglobin an intracellular oxygen-binding protein found in β-cells, inducible hypoxia. It our hypothesis that cytoglobin induction overexpression may improve survival function transplanted islets preventing ischemic cell death. Lewis rat MIN6 cells were transfected with gene. Control held 4 hours at 20% oxygen before glucose challenge. Another group was then 1% prior Untreated or ( n = 800) beneath renal capsule streptozotocin diabetic rats. In another study, Sprague-Dawley into Fasting blood used indicator survival. retained ability secrete insulin low concentrations contrast controls. over expression reduced development central necrosis 5 days tissue culture. inhibited onset immunorejection (14 ± 2 days) compared controls (5 days). useful adjunct transplantation.