Distinct faces of the Ku heterodimer mediate DNA repair and telomeric functions
作者： Albert Ribes-Zamora , Ivana Mihalek , Olivier Lichtarge , Alison A Bertuch
DOI: 10.1038/NSMB1214
关键词:
摘要: The Ku heterodimer, comprised of Ku70 and Ku80 subunits, is a conserved complex involved in nonhomologous end-joining (NHEJ). However, it also functions maintenance telomeres, chromosome termini normally resistant to events. To elucidate the spatial organization these functions, we rationally guided mutagenesis yeast with real-valued evolutionary trace (rvET). This revealed two ancestrally related α-helices: one on surface that required for NHEJ, second telomeric heterochromatin formation. When bound DNA end, containing NHEJ-specific helix oriented toward terminus, whereas function–specific faces inward, chromatin, when telomere. We propose 'two-face' model divergent evolution allowed Ku's dual role NHEJ telomere maintenance.
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