Transmembrane P-glycoprotein (P-gp/P-170) in HIV infection: analysis of lymphocyte surface expression and drug-unrelated function.
作者： M.B. LUCIA , R. CAUDA , A.L. LANDAY , W. MALORNI , G. DONELLI
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摘要: P-glycoprotein (P-gp/P-170), a transmembrane efflux pump known to be one of the mechanisms responsible for multidrug resistance in cancer therapy, is constitutively expressed several solid human tissues as well normal peripheral blood lymphocytes and bone marrow cells. In particular, this molecule has been associated with transport perforin other cytolysins natural killer (NK) T cytotoxic lymphocytes. present study, we analyzed (PBLs) from controls HIV+ patients phenotypic expression function P-gp/P-170 molecule. We found that 90% all PBL subsets (i.e., CD4+, CD8+, CD56+, CD19+ cells) surface both patients. However, significant decrease CD4+/P-170+ CD19+/P-170+ cells was observed individuals respect controls. PHA IL-2 stimulation PBLs unable increase patients, despite increased de...
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PM Chaudhary, EB Mechetner, IB Roninson, Expression and activity of the multidrug resistance P-glycoprotein in human peripheral blood lymphocytes [see comments] Blood. ,vol. 80, pp. 2735- 2739 ,(1992) , 10.1182/BLOOD.V80.11.2735.BLOODJOURNAL80112735
WT Klimecki, BW Futscher, TM Grogan, WS Dalton, P-glycoprotein expression and function in circulating blood cells from normal volunteers Blood. ,vol. 83, pp. 2451- 2458 ,(1994) , 10.1182/BLOOD.V83.9.2451.2451
M.M. Cornwell, I. Pastan, M.M. Gottesman, Certain calcium channel blockers bind specifically to multidrug-resistant human KB carcinoma membrane vesicles and inhibit drug binding to P-glycoprotein. Journal of Biological Chemistry. ,vol. 262, pp. 2166- 2170 ,(1987) , 10.1016/S0021-9258(18)61633-3
Yasuyuki Morishita, Shigeo Mori, Hirofumi Hamada, Isamu Sugawara, Takashi Tsuruo, Iyo Kataoka, Shinji Itoyama, Tissue Distribution of P-Glycoprotein Encoded by a Multidrug-resistant Gene as Revealed by a Monoclonal Antibody, MRK 16 Cancer Research. ,vol. 48, pp. 1926- 1929 ,(1988)
Z. Darzynkiewicz, F. Traganos, J. Kapuscinski, M. R. Melamed, L. Staiano-Coico, Interactions of Rhodamine 123 with Living Cells Studied by Flow Cytometry Cancer Research. ,vol. 42, pp. 799- 806 ,(1982)
Nandigama Krishnamachary, The MRP Gene Associated with a Non-P-glycoprotein Multidrug Resistance Encodes a 190-kDa Membrane Bound Glycoprotein Cancer Research. ,vol. 53, pp. 3658- 3661 ,(1993)
F. Thiebaut, T. Tsuruo, H. Hamada, M. M. Gottesman, I. Pastan, M. C. Willingham, Cellular localization of the multidrug-resistance gene product P-glycoprotein in normal human tissues Proceedings of the National Academy of Sciences of the United States of America. ,vol. 84, pp. 7735- 7738 ,(1987) , 10.1073/PNAS.84.21.7735
John S. Coon, Yazhang Wang, Steven D. Bines, Penelope N. Markham, Anita S.F. Chong, Howard M. Gebel, Multidrug resistance activity in human lymphocytes. Human Immunology. ,vol. 32, pp. 134- 140 ,(1991) , 10.1016/0198-8859(91)90110-U
Alexander A. Neyfakh, Use of fluorescent dyes as molecular probes for the study of multidrug resistance Experimental Cell Research. ,vol. 174, pp. 168- 176 ,(1988) , 10.1016/0014-4827(88)90152-8
Sastry Gollapudi, Sudhir Gupta, Human immunodeficiency virus I-induced expression of P-glycoprotein Biochemical and Biophysical Research Communications. ,vol. 171, pp. 1002- 1007 ,(1990) , 10.1016/0006-291X(90)90783-J