CtsR, a novel regulator of stress and heat shock response, controls clp and molecular chaperone gene expression in Gram‐positive bacteria

摘要: clpP and clpC of Bacillus subtillis encode subunits the Clp ATP-dependent protease are required for stress survival, including growth at high temperature. They play essential roles in stationary phase adaptive responses such as competence sporulation developmental pathways, belong to so-called class III group heat shock genes, whose mode regulation is unknown expression induced by or general conditions. The product ctsR, first gene operon, has now been shown act a repressor both clpC, well clpE, which encodes novel member Hsp100 ATPase family. CtsR protein was purified bind specifically promoter regions all three clp genes. Random mutagenesis, DNasel footprinting DNA sequence deletions comparisons were used define consensus recognition directly repeated heptad upstream from This target also found other genes several Gram-positive bacteria, Listeria monocytogenes, Streptococcus salivarius, S. pneumoniae, pyogenes, thermophilus, Enterococcus faecalis, Staphylococcus aureus, Leuconostoc oenos, Lactobacillus sake, Lactococcus lactis Clostridium acetobutylicum. homologues identified these indicating that highly conserved bacteria.