Two dimensional mapping of the perfusion of the retina and optic nerve head.

摘要: AIM--To present a new non-invasive method of performing high definition topography perfused vessels the retina and optic nerve head with simultaneous evaluation blood flow. METHOD--By combination laser Doppler flowmeter scanning system perfusion is visualised. The principles measuring flow by flowmetry are based on optical effect: light scattered moving particle shifted in frequency an amount delta f. Our data acquisition modified tomograph. technical retinal area measurement 2.7 mm x 0.7 mm, 10 degrees field 256 points 64 lines, accuracy microns, wavelength 670 nm 790 nm, power 100 microW 200 microW, time 2.048 s. Every line scanned 128 times sampling rate 4000 Hz. By discrete fast Fourier transformation over intensities each point shift calculated for point. With these two dimensional map created. brightness pixel coded value shift. Offline capillary estimated arbitrary units according to theory every region interest picture. We reliability validity method. Retinal was measured (SLDF) while varying intraocular pressure suction cup three healthy volunteers. Measurements performed 47 eyes presented were correlated gained commercially available flowmeter. Perfusion pictures superficial layer deep prelaminar layers presented. RESULTS--The coefficients r1 9flow9, 9volume9, 9velocity9 0.84, 0.85, 0.84 respectively. found significant linear relation between SLDF ocular (r = p