Mechanisms by which barbiturates suppress lymphocyte responses to phytohaemagglutinin stimulation

摘要: Certain barbiturates (the intermediate- and short-acting drugs) reduce the growth of PHA-stimulated peripheral blood mononuclear cells, but phenobarbitone (a long-acting drug) is ineffective: replicative more sensitive than activation. This action associated with a marked reduction in density interleukin-2 receptors on cell membrane numbers cells expressing them, rate cannot be restored by supplementation tissue culture medium exogenous interleukin-2. The affected show swelling mitochondria cytoplasmic fat globules: presence residual bodies myelin figures indicate an increase degradation organelles. There was no appreciable number necrotic seen electron microscopy cultures exposed to high concentrations thiopentone or other potent barbiturates, debris DNA cytogram at 72 h indicated some death suppressing barbiturates. It would inadvisable administer these short- intermediate-acting over long periods, particularly dosage, because potential danger iatrogenic immunosuppression.