Polyribosome metabolism in Escherichia coli treated with chloramphenicol, neomycin, spectinomycin or tetracycline.

摘要: Abstract When chloramphenicol, neomycin, spectinomycin or tetracycline is added to growing cells at levels sufficient block nearly all protein synthesis, polyribosome formation continues: pulse-labeled mRNA still joins ribosomes rates comparable that in untreated cells. The accumulates polyribosomes until there several-fold more than Ribosomes these seem be 30 s-50 s ribosome couples, each bearing two tRNA molecules. In control studies carried out during and after cell lysis, neither cellular RNA nor f2 phage joined efficiently the absence of an source energy. Thus, probably takes place drug-treated cultures not artifact lysis. We propose antibiotics uncouple from peptide bond formation. Movement along could continue based on apposition molecules corresponding codons. Each successive aminoacyl-tRNA would transiently reside peptidyl-tRNA site ribosome.