Substance P-induced augmentation of cutaneous vascular permeability and granulocyte infiltration in mice is mast cell dependent.

摘要: The undecapeptide substance P is thought to mediate both vasodilatation and augmented vascular permeability when released from sensory nerve endings in the skin. Substance also induces mast cell degranulation vitro or vivo. However, extent which P-induced changes are cell-dependent unclear. We investigated this issue by injecting certain related peptides (substance P1-4, P4-11) into skin of genetically cell-deficient WBB6F1-W/W WCB6F1- SI/SId mice congenic normal (+/+) mice, W/W had undergone selective local repair their deficiency intradermal injection IL-3-dependent cells generated bone marrow +/+ mice. induced significant augmentation cutaneous swelling injected at doses as low 2 pmol i.d. granulocyte infiltration, although infiltrate were modest seen peptide 5 more than 20-fold higher those required for induction tissue swelling. effects on swelling, permeability, infiltration virtually entirely dependent. By contrast, P1-4 was inactive our assays 25 nmol/site, P4-11 least part independent.